Authored By: Archana Rani & Yogendra Singh
ISBN: 9789367551776
Binding: Paperback
Year of Publishing: 2026
Imprint: CONTENT VIBES
Pages: 200
Language: English
Length: 152
Breadth: 18
Height: 229
Weight: 290
Designed for fast revision, this book distills recombinant DNA technology into high-yield notes, flowcharts, and comparison tables exactly the way competitive exams ask it (ICAR PG/PhD, ASRB-NET/ARS Pre, SAU entrances, GATE-BT/XL basics).
Why it helps you score:
One-page concept maps for each unit: enzymes → vectors → cloning → screening → analysis.
Must-know tables: Type I/II/IIS REs, recognition sites, overhangs; plasmid/cosmid/BAC/PAC/YAC features; selectable/reporters.
Workflow cheatsheets: competent cell prep, transformation (heat-shock/electroporation), cloning pipeline, library construction, probe prep.
Numerical boxes: Tm, insert:vector molar ratios, PCR copy number, gel band size estimation.
Question bank: unit-wise MCQs (single-best, numerical), Assertion–Reason, Match-the-pairs, figure-based gel/blot questions, with keys & brief explanations.
Troubleshooting cues: no colonies, smeared gels, non-specific PCR, false positives in blue-white screening.
1 Recombinant DNA Technology
2 Restriction Endonucleases: Types and Uses
3 DNA Manipulating Enzymes, DNA Ligases
4 Vectors: Properties of an Ideal Vector, Structure of Vector
5 Cloning Vectors and Expression Vectors
6 Plasmids, Cosmids, Phagemids
7 Bacs, Pacs, Yacs, Transposon Vectors, Shuttle Vectors, Co-Integrating Vectors
8 Competent Cells
9 Gene Isolation and Cloning
10 Genetic Transformation of E. Coli, Gel Electrophoresis
11 Preparation of Probes
12 Southern Blotting; Northern Blotting; Western Blotting
13 PCR and PCR Based Methods in Recombinant DNA Technology
Dr. Archana Rani is currently serving as a Senior Research Fellow in the Department of Plant Breeding and Genetics at Jawaharlal Nehru Krishi Vishwa Vidyalaya (JNKVV), Jabalpur, Madhya Pradesh. She holds both her Master’s and Doctoral degrees in Genetics from Rajendra Prasad Central Agricultural University, Pusa, Samastipur, Bihar, where she was awarded university fellowships for academic excellence throughout her postgraduate studies.
With nearly a decade of professional experience in agricultural research and academics, Dr. Rani has significantly contributed to several key research projects, including a Japan –funded JICA project on the Evaluation of promising soybean genotypes under excessive soil moisture conditions and the ICAR-funded CRP on Hybrid Technology in Wheat. Her teaching and research engagements have also extended to U.P. College (affiliated with LNM University, Darbhanga) and her alma mater in Pusa, Bihar.
A major milestone in her career is her association with the development of the wheat variety MP 3535. Her scholarly contributions are substantial, with over 30 research papers, 19 book chapters, and 27 extension articles published in reputed platforms. She is also the author of the academic book Agricultural Biotechnology – Concept and Practices, which reflects her expertise in the field. In recognition of her scientific contributions, Dr. Rani was honored with the Young Scientist Award at an international symposium held at Sido Kanhu Murmu University, Dumka, Jharkhand. Her active participation in 17 national and international seminars, symposia, and training programs further underscores her commitment to continuous learning and knowledge dissemination in the agricultural sciences.
Yogendra Singh: Senior Assistant Professor (Biotechnology), Department of Genetics and Plant Breeding, Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur-482004, Madhya Pradesh