Authored By: Archana Rani & Yogendra Singh
ISBN: 9789367557891
Binding: Paperback
Year of Publishing: 2026
Imprint: CONTENT VIBES
Pages: 138
Language: English
Length: 152
Breadth: 17
Height: 229
Weight: 210
This lab-focused manual converts your 10 practicals into step-by-step SOPs with checklists, calculations, controls, and troubleshooting—exactly what exams and vivas look for. Each exercise (growth media → buffers → miniprep → QC → digestion → gel → competent cells → E. coli transformation → screening → confirmation) includes:
Materials & prep tables (reagent recipes, stock→working dilutions, pH setpoints).
Critical parameters (temperatures, times, rpm, enzyme units, percent agarose).
Controls (positive/negative, vector-only, undigested DNA).
Result criteria (acceptance ranges: A260/280, band sizes, colony patterns).
Viva boxes (why steps work: alkaline lysis chemistry, star activity, blue-white logic).
Troubleshooting maps (no colonies, smeared gels, low DNA yield, non-specific PCR).
Mini-numericals appear where needed: A260 quant, insert:vector molar ratio, digestion setup, spray-and-plate volumes, and transformation efficiency.
1. Preparation of Growth, Madia
2 Preparation of Stock Solutions and Buffers
3 Plasmid DNA Isolation
4 Quality and Quantity Assessment of DNA
5 Restriction Digestion of DNA
6 Agarose Gel Electrophoresis
7 Preparation of Competent Cells
8 Preparation of Genetic Transformation of E. Coli
9 Screening of Recombinant DNA Clones in E. Coli.
10 Confirmation of Recombinant Clones
Dr. Archana Rani is currently serving as a Senior Research Fellow in the Department of Plant Breeding and Genetics at Jawaharlal Nehru Krishi Vishwa Vidyalaya (JNKVV), Jabalpur, Madhya Pradesh. She holds both her Master’s and Doctoral degrees in Genetics from Rajendra Prasad Central Agricultural University, Pusa, Samastipur, Bihar, where she was awarded university fellowships for academic excellence throughout her postgraduate studies.
With nearly a decade of professional experience in agricultural research and academics, Dr. Rani has significantly contributed to several key research projects, including a Japan –funded JICA project on the Evaluation of promising soybean genotypes under excessive soil moisture conditions and the ICAR-funded CRP on Hybrid Technology in Wheat. Her teaching and research engagements have also extended to U.P. College (affiliated with LNM University, Darbhanga) and her alma mater in Pusa, Bihar.
A major milestone in her career is her association with the development of the wheat variety MP 3535. Her scholarly contributions are substantial, with over 30 research papers, 19 book chapters, and 27 extension articles published in reputed platforms. She is also the author of the academic book Agricultural Biotechnology – Concept and Practices, which reflects her expertise in the field. In recognition of her scientific contributions, Dr. Rani was honored with the Young Scientist Award at an international symposium held at Sido Kanhu Murmu University, Dumka, Jharkhand. Her active participation in 17 national and international seminars, symposia, and training programs further underscores her commitment to continuous learning and knowledge dissemination in the agricultural sciences.
Yogendra Singh: Senior Assistant Professor (Biotechnology), Department of Genetics and Plant Breeding, Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur-482004, Madhya Pradesh